lmnb1 3  (Addgene inc)

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: Control of post-transcriptional LMNB1 overexpression and transfection efficiency in LoVo cell line. (A) Western blot analysis of post-translational expression LMNB1 in LoVo cell line with and without overexpression of LMNB1 after treatment of cells with 5-FU. (B) Relative to GADPH densitometric analysis of LMNB1 reactive bands in transfected LoVo cells without upregulated LMNB1 and with cells upregulated LMNB1 following treatment with 5-FU. (C) The analysis of fluorescence of LMNB1 in LoVo cell line with and without overexpression of LMNB1 after treatment with 1 mM 5-FU. (D) Fluorescence intensity analysis of LMNB1 in LoVo cell line with and without overexpression of LMNB1. Bar, 50 μ m. $ Statistically significant differences between cells without upregulated LMNB1 and with cells upregulated LMNB1. The results are presented as the means ± SEM ( * P<0.05).

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Over Expression, Transfection, Western Blot, Expressing, Fluorescence

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: The quantitative analysis of viable, apoptosis and necrosis in LoVo cells with and without overexpression of LMNB1 after treatment with 5-FU. (A) Percentage of viable cells without upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (B) Percentage of viable cells with upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (C) Comparison of percentage of viable cells without upregulated LMNB1 and with upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (D) Percentage of apoptotic cells without upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (E) Percentage of apoptotic cells with upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (F) Comparison of percentage of apoptotic cells without upregulated LMNB1 and with upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (G) Percentage of necrotic cells without upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (H) Percentage of necrotic cells with upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. (I) Comparison of percentage of necrotic cells without upregulated LMNB1 and with upregulated LMNB1 in control cells and treated with 0.1, 1 and 5 mM 5-FU. * Statistically significant differences between cells treated with 5-FU in comparison to the control in a group of the cell without upregulated LMNB1, P<0.05. # Statistically significant differences within cells with upregulated LMNB1, P<0.05. $ Statistically significant differences between cells without upregulated LMNB and with upregulated LMNB1, P<0.05.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Over Expression

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: The quantitative analysis of cell cycle in LoVo cells with and without overexpression of LMNB1 after treatment with 5-FU. (A) Percentage of cells in sub-G1 phase. (B) Percentage of cells in G0/G1 phase. (C) Percentage of cells in S phase. (D) Percentage of cells in G2/M phase. (E) Percentage of cells with DNA content >4N. * Statistically significant differences between cells treated with 5-FU in comparison to the control in a group of the cell without upregulated LMNB1, P<0.05. # Statistically significant differences within cells with upregulated LMNB1, P<0.05. $ Statistically significant differences between cells without upregulated LMNB1 and with upregulated LMNB1, P<0.05.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Over Expression

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: Fluorescence analysis of β-tubulin (red) and F-actin (green) organization in LoVo cells with and without overexpression of LMNB1 in control cells and after treatment with 0.1, 1 and 5 mM 5-FU. Nuclei were counterstained with DAPI. Bar, 50 μ m.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Fluorescence, Over Expression

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: Fluorescence analysis of F-actin organization and fluorescence intensity of nuclear F-actin in LoVo cells. (A) Fluorescence analysis of F-actin organization in LoVo cells with and without overexpression of LMNB1 in control cells and after treatment with 0.1, 1 and 5 mM 5-FU. Nuclei were counterstained with DAPI. Bar, 50 μ m. (B) Fluorescence intensity of nuclear F-actin in LoVo cells with and without overexpression of LMNB1 in control cells and after treatment with 0.1, 1 and 5 mM 5-FU. * Statistically significant differences between cells treated with 5-FU in comparison to the control in a group of the cell without upregulated LMNB1, P<0.05. # Statistically significant differences within cells with upregulated LMNB1, P<0.05. $ Statistically significant differences between cells without upregulated LMNB1 and with upregulated LMNB1, P<0.05.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Fluorescence, Over Expression

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: Fluorescence analysis of β-catenin and F-actin and fluorescence intensity of β-catenin measured in nuclear and cell-cell interaction areas in LoVo cells. (A) Fluorescence analysis of β-catenin (green) and F-actin (red) organization in LoVo cells with and without overexpression of LMNB1 in control cells and after treatment with 0.1, 1 and 5 mM 5-FU. Nuclei were counterstained with DAPI. Bar, 50 µ m. (B) Fluorescence intensity of β-catenin measured in nuclear and (C) cell-cell interaction areas in LoVo cells with and without overexpression of LMNB1 in control cells and after treatment with 0.1, 1 and 5 mM 5-FU. * Statistically significant differences between cells treated with 5-FU in comparison to the control in a group of the cell without upregulated LMNB1, P<0.05. # Statistically significant differences within cells with upregulated LMNB1, P<0.05. $ Statistically significant differences between cells without upregu-lated LMNB1 and with upregulated LMNB1, P<0.05.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Fluorescence, Over Expression

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: Fluorescence analysis of lamin A/C (red) and F-actin (green) organization in LoVo cells with and without overexpression of LMNB1 in control cells and after treatment with 0.1, 1 and 5 mM of 5-FU. Nuclei were counterstained with DAPI. Bar, 50 µ m.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Fluorescence, Over Expression

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: Wound healing analysis. (A) The time-course of closure of the wounded areas in LoVo cells without upregulated LMNB1 in control cells and after treatment with 5-FU. (B) The time-course of closure of the wounded areas in LoVo cells with upregulated LMNB1 in control cells and after treatment with 5-FU. (C) Wound closure after 24 h in LoVo cells without and with upregulated LMNB1 in control cells and after treatment with 5-FU. * Statistically significant differences between cells treated with 5-FU in comparison to the control in a group of the cell without upregulated LMNB1, P<0.05. # Statistically significant differences within cells with upregulated LMNB1, P<0.05. $ Statistically significant differences between cells without upregulated LMNB1 and with upregulated LMNB1, P<0.05. (D) Representative images of LoVo cells without and with upregulated LMNB1 after treatment with 0.1 mM of 5-FU.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques:

Journal: International Journal of Oncology

Article Title: Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

doi: 10.3892/ijo.2017.4182

Figure Lengend Snippet: LMNB1 mRNA expression in patients with colorectal adenocarcinoma. (A) Low LMNB1 mRNA expression associated with lower overall survival in the first 30 months of disease. (B) No significant differences in overall survival in longer perspective. Probabilities of overall survival of total 188 patients with colorectal adenocarcinoma were analyzed by Kaplan-Meier survival analysis.

Article Snippet: After trypsinization, the cells were transfected with using the SE Cell Line 4D-Nucleofector™ X kit and 2 μ g human the cDNA of LMNB1 cloned into pCMV6-XL4 expression plasmid vector (NM_005573; OriGene, Rockville, MD, USA) according to the manufacturer's instructions.

Techniques: Expressing